GNAI1 Polyclonal antibody

GNAI1 Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12617-1-AP

Synonyms

Gi, GNAI1

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Validation Data Gallery

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  WB analysis using 12617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 12617-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12617-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 12617-1-AP

  IP result of anti-GNAI1 (IP:12617-1-AP, 4ug; Detection:12617-1-AP 1:1000) with SH-SY5Y cells lysate 800 ug.

• 

  FC experiment of SH-SY5Y using 12617-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.20 ug/test Anti-Human GNAI1 (12617-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue, rat brain tissue
• Positive IP detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

12617-1-AP targets GNAI1 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNAI1 fusion protein Ag3290
• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
• Calculated molecular weight: 354 aa, 40 kDa
• Observed molecular weight: 40 kDa
• GenBank accession number: BC026326
• Gene symbol: GNAI1
• Gene ID (NCBI): 2770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Drug Des Devel Ther; Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.; Authors - Dianna Liu
human	WB	J Oncol; Characterization of the Newly Established Homoharringtonine- (HHT-) Resistant Cell Lines and Mechanisms of Resistance; Authors - Fenglin Li