|Positive WB detected in||mouse brain tissue, fetal human brain tissue, rat brain tissue|
|Positive IP detected in||SH-SY5Y cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
12617-1-AP targets GNAI1 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GNAI1 fusion protein Ag3290|
|Full Name||guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1|
|Calculated molecular weight||354 aa, 40 kDa|
|Observed molecular weight||38-40 kDa|
|GenBank accession number||BC026326|
|Gene ID (NCBI)||2770|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.
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