GNAI1 Polyclonal antibody

GNAI1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12617-1-AP



Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12617-1-AP targets GNAI1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNAI1 fusion protein Ag3290
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Calculated molecular weight 354 aa, 40 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC026326
Gene symbol GNAI1
Gene ID (NCBI) 2770
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.


Product Specific Protocols
WB protocol for GNAI1 antibody 12617-1-APDownload protocol
IHC protocol for GNAI1 antibody 12617-1-APDownload protocol
IP protocol for GNAI1 antibody 12617-1-APDownload protocol
FC protocol for GNAI1 antibody 12617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Drug Des Devel Ther

Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.

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Characterization of the Newly Established Homoharringtonine- (HHT-) Resistant Cell Lines and Mechanisms of Resistance

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