GNAI2 Monoclonal antibody, PBS Only

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  WB analysis using 67007-1-Ig (same clone as 67007-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67007-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 67007-1-Ig (same clone as 67007-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67007-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67007-1-Ig (same clone as 67007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 67007-1-Ig (GNAI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67007-1-PBS in a different storage buffer formulation.

GNAI2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67007-1-PBS

Clone No.3F6H5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

3F6H5, Adenylate cyclase-inhibiting G alpha protein, GIP, GNAI2B, Guanine nucleotide-binding protein G(i) subunit alpha-2

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Product Information

67007-1-PBS targets GNAI2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28560

  Product name: Recombinant human GNAI2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-355 aa of BC012138

  Sequence: MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC012138
• Gene Symbol: GNAI2
• Gene ID (NCBI): 2771
• RRID: AB_2882324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P04899
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.