GNAI3 Recombinant monoclonal antibody

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• GNAI3 Recombinant monoclonal antibody (88438-1-RR)

GNAI3 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88438-1-RR

Clone No.261052B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

87U6, G(i) alpha-3, Guanine nucleotide-binding protein G(i) subunit alpha-3

• 

  WB analysis using 88438-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88438-1-RR (GNAI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 88438-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GNAI3 antibody (88438-1-RR, Clone: 261052B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, U-937 cells, A431 cell, mouse kidney tissue, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88438-1-RR targets GNAI3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2229

  Product name: Recombinant human GNAI3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-354 aa of BC025285

  Sequence: MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC025285
• Gene Symbol: GNAI3
• Gene ID (NCBI): 2773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08754
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)