GNAI3 Recombinant monoclonal antibody, PBS Only (Detector)

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• GNAI3 Recombinant monoclonal antibody, PBS Only (Detector) (88438-3-PBS)

GNAI3 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 88438-3-PBS

Clone No.261052A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

• 

  Sandwich ELISA sample test of MP03552-1

  The mean GNAI3 concentration was determined to be 305.39 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load, 345.35 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load and 217.31 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA standard curve of MP03552-1

  Sandwich ELISA standard curve of MP03552-1, Human GNAI3 Recombinant Matched Antibody Pair - PBS only. 88438-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2229. 88438-3-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array sample test of MP03552-1

  The mean GNAI3 concentration was determined to be 90.7 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load, 68.8 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, 54.5 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array standard curve of MP03552-1

  Cytometric bead array standard curve of MP03552-1, GNAI3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88438-4-PBS. Detection antibody: 88438-3-PBS. Standard: Ag2229. Range: 0.625-80 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88438-3-PBS targets GNAI3 as part of a matched antibody pair:

MP03552-1: 88438-4-PBS capture and 88438-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2229

  Product name: Recombinant human GNAI3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-354 aa of BC025285

  Sequence: MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
• Calculated Molecular Weight: 40 kDa
• GenBank Accession Number: BC025285
• Gene Symbol: GNAI3
• Gene ID (NCBI): 2773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08754
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.