|Positive WB detected in||rat brain tissue, human brain tissue, human liver tissue, mouse testis tissue, Y79 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12635-1-AP targets GNAO1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GNAO1 fusion protein Ag3330|
|Full Name||guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O|
|Calculated molecular weight||302 aa, 35 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC030027|
|Gene ID (NCBI)||2775|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The down-regulation of GNAO1 and its promoting role in hepatocellular carcinoma.
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Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis.
DNMT1 mediated promoter methylation of GNAO1 in hepatoma carcinoma cells.
Efficacy of Hybrid Tetrahydrobenzo[d]thiazole Based Aryl Piperazines D-264 and D-301 at D₂ and D₃ Receptors.