GNAO1 Polyclonal antibody

GNAO1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 12635-1-AP

Synonyms

G ALPHA o, GNAO, GNAO1



Tested Applications

Positive WB detected inmouse testis tissue, rat brain tissue, Y79 cells, human liver tissue, human brain tissue, rat testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12635-1-AP targets GNAO1 in WB, IP, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNAO1 fusion protein Ag3330
Full Name guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Calculated molecular weight 302 aa, 35 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC030027
Gene symbol GNAO1
Gene ID (NCBI) 2775
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.

Protocols

Product Specific Protocols
WB protocol for GNAO1 antibody 12635-1-APDownload protocol
IHC protocol for GNAO1 antibody 12635-1-APDownload protocol
IP protocol for GNAO1 antibody 12635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biosci Rep

The down-regulation of GNAO1 and its promoting role in hepatocellular carcinoma.

Authors - Pei Xiaoyu X
humanIHC

Int J Mol Med

Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis.

Authors - Zhen Liu
humanWB

Gene

DNMT1 mediated promoter methylation of GNAO1 in hepatoma carcinoma cells.

Authors - Diannan Xu
humanWB

Neurochem Res

Efficacy of Hybrid Tetrahydrobenzo[d]thiazole Based Aryl Piperazines D-264 and D-301 at D₂ and D₃ Receptors.

Authors - Juan Zhen