Home
Products
Primary Antibodies
GNAO1 Monoclonal antibody, PBS Only (Capture) (68027-1-PBS)

GNAO1 Monoclonal antibody, PBS Only (Capture)

Name: GNAO1 Monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 68027-1-PBS-100UG

GNAO1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 68027-1-PBS

Clone No. 1B5G2

Review Product

Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

1B5G2, G ALPHA o, GNAO, Guanine nucleotide-binding protein G(o) subunit alpha

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHCIF-PCytometric bead array sample testCytometric bead array

WB analysis using 68027-1-Ig (same clone as 68027-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68027-1-Ig (GNAO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of rat cerebellum using 68027-1-Ig (same clone as 68027-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using GNAO1 antibody (68027-1-Ig, Clone: 1B5G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP51895-1
Cytometric bead array sample test of MP51895-1,GNAO1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68027-1-PBS. Detection antibody: 68027-2-PBS.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP51895-1
Cytometric bead array standard curve of MP51895-1, GNAO1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68027-1-PBS. Detection antibody: 68027-2-PBS. Standard:Ag32045. Range: 1.563-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for GNAO1 in ?

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

68027-1-PBS targets GNAO1 as part of a matched antibody pair:

MP51895-1: 68027-1-PBS capture and 68027-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig, rabbit, chicken
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag32045 Product name: Recombinant human GNAO1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-302 aa of BC030027 Sequence: MKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGPNTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY Predict reactive species
Full Name	guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Calculated Molecular Weight	302 aa, 35 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	BC030027
Gene Symbol	GNAO1
Gene ID (NCBI)	2775
RRID	AB_2918770
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P09471
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.