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  • KD/KO Validated

GNAQ Polyclonal antibody

GNAQ Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13927-1-AP

Synonyms

G ALPHA q, GAQ, GNAQ



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, mouse lung tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13927-1-AP targets GNAQ in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNAQ fusion protein Ag4947
Full Name guanine nucleotide binding protein (G protein), q polypeptide
Calculated molecular weight 42 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC057777
Gene symbol GNAQ
Gene ID (NCBI) 2776
RRIDAB_2111647
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Protocols

Product Specific Protocols
WB protocol for GNAQ antibody 13927-1-APDownload protocol
IHC protocol for GNAQ antibody 13927-1-APDownload protocol
IF protocol for GNAQ antibody 13927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Exp Med

Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells.

Authors - Miwa Sasai
mouseWB,IF

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang
mouseWB

Oncogene

Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.

Authors - Yongyun Li
humanWB

Proteomics

iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells.

Authors - Hao Chen
humanWB,IHC

Oncol Rep

G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways.

Authors - Yizhuo Wang
  • KD Validated
humanWB,IF

J Biol Chem

Enhanced membrane binding of oncogenic G protein αqQ209L confers resistance to inhibitor YM-254890

Authors - Clinita E Randolph