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GNAQ Polyclonal antibody
GNAQ Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 13927-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, human brain tissue, mouse lung tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
13927-1-AP targets GNAQ in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GNAQ fusion protein Ag4947 |
Full Name | guanine nucleotide binding protein (G protein), q polypeptide |
Calculated molecular weight | 42 kDa |
Observed molecular weight | 38-40 kDa |
GenBank accession number | BC057777 |
Gene symbol | GNAQ |
Gene ID (NCBI) | 2776 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for GNAQ antibody 13927-1-AP | Download protocol |
IHC protocol for GNAQ antibody 13927-1-AP | Download protocol |
IF protocol for GNAQ antibody 13927-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncol Rep G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways.
| ||
Proteomics iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells. | ||
Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. | ||
J Exp Med Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells. |