GNAQ Polyclonal antibody

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• GNAQ Polyclonal antibody (13927-1-AP)

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• KD/KO Validated

GNAQ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13927-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q, GAQ, G ALPHA q, CMC1

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  WB analysis using 13927-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 13927-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse lung using 13927-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skin using 13927-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 13927-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GNAQ antibody (13927-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 13927-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GNAQ antibody (13927-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse lung tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human testis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13927-1-AP targets GNAQ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4947

  Product name: Recombinant human GNAQ protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-359 aa of BC057777

  Sequence: MTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

• Full Name: guanine nucleotide binding protein (G protein), q polypeptide
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC057777
• Gene Symbol: GNAQ
• Gene ID (NCBI): 2776
• RRID: AB_2111647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50148
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Publications

What published studies show

Anti-GNAQ antibody (Cat# 13927-1-AP) has 13 citations published in journals including J Exp Med, Oncogene, J Biol Chem, FASEB J, Proteomics, Oncol Rep, Biomedicines, ACS Pharmacol Transl Sci, Ecotoxicol Environ Saf, Biochem Pharmacol, Toxicol Appl Pharmacol, and Elife. Research fields span T cell signaling, uveal melanoma, gastric cancer, acute kidney injury, GPCR coupling, ovarian injury, colon cancer proteomics, and male fertility.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Exp Med; Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells.; Authors - Miwa Sasai
mouse	WB	Oncogene; Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.; Authors - Yongyun Li
rat	WB	Ecotoxicol Environ Saf; Protective role of RGS2 in PM2.5-induced ovarian injury in rats: Modulation of Gq/11 signaling to maintain granulosa cell [Ca2 + ]i stability; Authors - Zhenhua Ma
		Biochem Pharmacol; Sodium butyrate upregulates URAT1-mediated urate reabsorption in HK2 cells by activating FFAR2/p38 MAPK/ELK1/ARRB2 axis and relieving NF-κB-HNF1β competitive binding.; Authors - Cheng Cao
human	WB,IHC	Oncol Rep; G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways.; Authors - Yizhuo Wang ; KD Validated
		Biomedicines; Combination of Three Herbal Components (ISL, Que, Meth) Suppresses Uveal Melanoma Growth via Gαq/MEK/YAP Axis Modulation and Apoptosis.; Authors - Xiqianru Zhang