GNB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GNB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 10247-2-AP

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, GBB1

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Validation Data Gallery

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  WB analysis using 10247-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10247-2-AP (GNB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10247-2-AP (GNB1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10247-2-AP (GNB1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10247-2-AP (GNB1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 10247-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB1 antibody (10247-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below

Product Information

10247-2-AP targets GNB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNB1 fusion protein Ag0313
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC004186
• Gene Symbol: GNB1
• Gene ID (NCBI): 2782
• RRID: AB_2111820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

Publications

Species	Application	Title
mouse	WB,IF,IP	Cell; Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.; Authors - Gonzalo P Solis ; KD Validated
human	IF,IP	PLoS Pathog; H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humans.; Authors - Fangtao Li
mouse	WB	Neuropharmacology.; A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.; Authors - Saba LM
mouse	IHC	Exp Eye Res; Nr2e3-directed transcriptional regulation of genes involved in photoreceptor development and cell-type specific phototransduction.; Authors - Haider Neena B NB
mouse	WB	Dev Dyn; Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.; Authors - Okae Hiroaki H
human	WB	Bioengineered; Shikonin induces cell autophagy via modulating the microRNA -545-3p/guanine nucleotide binding protein beta polypeptide 1 axis, thereby disrupting cellular carcinogenesis in colon cancer.; Authors - ZhiWei Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nick (Verified Customer) (04-20-2022)

If I could give this three thumbs up, I would.

• Applications: Western Blot