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GNB1 Polyclonal antibody

GNB1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10247-2-AP

Synonyms

GNB1, Transducin beta chain 1



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10247-2-AP targets GNB1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB1 fusion protein Ag0313
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 1
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC004186
Gene symbol GNB1
Gene ID (NCBI) 2782
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

Protocols

Product Specific Protocols
WB protocol for GNB1 antibody 10247-2-APDownload protocol
IHC protocol for GNB1 antibody 10247-2-APDownload protocol
IF protocol for GNB1 antibody 10247-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dev Dyn

Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.

Authors - Okae Hiroaki H
mouseIHC

Exp Eye Res

Nr2e3-directed transcriptional regulation of genes involved in photoreceptor development and cell-type specific phototransduction.

Authors - Haider Neena B NB
mouseWB

Neuropharmacology.

A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.

Authors - Saba LM
mouseWB,IF,IP

Cell

Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.

Authors - Gonzalo P Solis
  • KD Validated