|Positive WB detected in||Jurkat cells, HeLa cells, mouse brain tissue|
|Positive IHC detected in||human pancreas cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10247-2-AP targets GNB1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GNB1 fusion protein Ag0313|
|Full Name||guanine nucleotide binding protein (G protein), beta polypeptide 1|
|Calculated molecular weight||37 kDa|
|Observed molecular weight||37 kDa|
|GenBank accession number||BC004186|
|Gene ID (NCBI)||2782|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).
Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.
Exp Eye Res
Nr2e3-directed transcriptional regulation of genes involved in photoreceptor development and cell-type specific phototransduction.
A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.
Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.