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  • KD/KO Validated

GNB1 Polyclonal antibody

GNB1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10247-2-AP

Synonyms

GNB1, Transducin beta chain 1



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10247-2-AP targets GNB1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB1 fusion protein Ag0313
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC004186
Gene Symbol GNB1
Gene ID (NCBI) 2782
RRIDAB_2111820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

Protocols

Product Specific Protocols
WB protocol for GNB1 antibody 10247-2-APDownload protocol
IHC protocol for GNB1 antibody 10247-2-APDownload protocol
IF protocol for GNB1 antibody 10247-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IP

Cell

Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.

Authors - Gonzalo P Solis
  • KD Validated
humanIF,IP

PLoS Pathog

H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humans.

Authors - Fangtao Li
mouseWB

Neuropharmacology.

A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.

Authors - Saba LM
mouseIHC

Exp Eye Res

Nr2e3-directed transcriptional regulation of genes involved in photoreceptor development and cell-type specific phototransduction.

Authors - Haider Neena B NB
mouseWB

Dev Dyn

Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.

Authors - Okae Hiroaki H
humanWB

Bioengineered

Shikonin induces cell autophagy via modulating the microRNA -545-3p/guanine nucleotide binding protein beta polypeptide 1 axis, thereby disrupting cellular carcinogenesis in colon cancer.

Authors - ZhiWei Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nick (Verified Customer) (04-20-2022)

If I could give this three thumbs up, I would.

  • Applications: Western Blot