GNB3 Polyclonal antibody

GNB3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10081-1-AP

Synonyms

GNB3, Transducin beta chain 3



Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, mouse eye tissue, mouse embryo tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10081-1-AP targets GNB3 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB3 fusion protein Ag0127
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 3
Calculated molecular weight 37 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC000115
Gene symbol GNB3
Gene ID (NCBI) 2784
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

Protocols

Product Specific Protocols
WB protocol for GNB3 antibody 10081-1-APDownload protocol
IHC protocol for GNB3 antibody 10081-1-APDownload protocol
IF protocol for GNB3 antibody 10081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

J Biol Chem

Pathogenic Mutations in Retinitis Pigmentosa 2 Predominantly Result in Loss of RP2 Protein Stability in Human and Zebrafish.

Authors - Fei Liu