GNB3 Polyclonal antibody

GNB3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10081-1-AP

Synonyms

GNB3, Transducin beta chain 3

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Validation Data Gallery

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  WB analysis of mouse brain using 10081-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 10081-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 10081-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10081-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 10081-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10081-1-AP (GNB3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 10081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10081-1-AP (GNB3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10081-1-AP

  Immunofluorescent analysis of HepG2 cells using 10081-1-AP (GNB3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, mouse eye tissue, mouse embryo tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 2 publications below

Product Information

10081-1-AP targets GNB3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNB3 fusion protein Ag0127
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC000115
• Gene Symbol: GNB3
• Gene ID (NCBI): 2784
• RRID: AB_2263264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

Publications

Species	Application	Title
mouse	IF	Elife; Injury-induced pulmonary tuft cells are heterogenous, arise independent of key Type 2 cytokines, and are dispensable for dysplastic repair; Authors - Justinn Barr
zebrafish	WB	Hum Mol Genet; CERKL gene knockout disturbs photoreceptor outer segment phagocytosis and causes rod-cone dystrophy in zebrafish.; Authors - Shanshan Yu
zebrafish	WB	J Biol Chem; Pathogenic Mutations in Retinitis Pigmentosa 2 Predominantly Result in Loss of RP2 Protein Stability in Human and Zebrafish.; Authors - Fei Liu
rat	WB	Sci Rep; PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.; Authors - Jing Li
human	IF	Nat Commun; The immune landscape of human thymic epithelial tumors; Authors - Zhongwei Xin
human	WB	Acta Neuropathol Commun; Synaptic proteomics reveal distinct molecular signatures of cognitive change and C9ORF72 repeat expansion in the human ALS cortex; Authors - Zsofia I. Laszlo