GNB5 Polyclonal antibody

• Featured Product
• KD/KO Validated

GNB5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11045-2-AP

Synonyms

GB5, Gbeta5, GNB5, Transducin beta chain 5

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 11045-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11045-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 11045-2-AP

  IP result of anti-GNB5 (IP:11045-2-AP, 4ug; Detection:11045-2-AP 1:500) with rat brain tissue lysate 4000ug.

• 

  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11045-2-AP (GNB5 antibody) at dilution of 1:100 (under 10x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

11045-2-AP targets GNB5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNB5 fusion protein Ag1518
• Full Name: guanine nucleotide binding protein (G protein), beta 5
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC011671
• Gene Symbol: GNB5
• Gene ID (NCBI): 10681
• RRID: AB_2109639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.

Publications

Species	Application	Title
human	WB	Oncogene; The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.; Authors - D Fuchs ; KD Validated
rat	WB	J Cell Mol Med; Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring.; Authors - Wenna Zhang
mouse	WB	Neuropharmacology.; A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.; Authors - Saba LM
mouse	WB, IF	PMC Biophys; Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling.; Authors - Christopher M Tracy
human	WB	FEBS J; PMID: 30719834; Authors - Sang-Min Park ; KD Validated
rat	WB	MedComm (2020); Transcriptomic analysis of rat brain response to alternating current electrical stimulation: unveiling insights via single-nucleus RNA sequencing; Authors - Yan Wang