Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11045-2-AP targets GNB5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1518 Product name: Recombinant human GNB5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-126 aa of BC011671 Sequence: MATEGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKVRHCSQPRYRGFRIPAYLKVLWSSCPALS Predict reactive species |
| Full Name | guanine nucleotide binding protein (G protein), beta 5 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39-42 kDa |
| GenBank Accession Number | BC011671 |
| Gene Symbol | GNB5 |
| Gene ID (NCBI) | 10681 |
| RRID | AB_2109639 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14775 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GNB5 antibody 11045-2-AP | Download protocol |
| IP protocol for GNB5 antibody 11045-2-AP | Download protocol |
| WB protocol for GNB5 antibody 11045-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GNB5 Polyclonal antibody (Cat# 11045-2-AP) has 6 total citations. These appear in journals including MedComm (2020), Oncogene, Neuropharmacology, J Cell Mol Med, FEBS J, and PMC Biophys. The research fields span neuroscience (brain electrical stimulation, GABAergic transmission), oncology (colon carcinoma, colorectal cancer resistance), cardiovascular biology (prenatal hypoxia effects on smooth muscle), and retinal cell signaling. All citations used WB as the primary application, with one also employing IF.
| Species | Application | Title |
|---|---|---|
MedComm (2020) Transcriptomic analysis of rat brain response to alternating current electrical stimulation: unveiling insights via single-nucleus RNA sequencing | ||
Oncogene The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.
| ||
J Cell Mol Med Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring. | ||
Neuropharmacology. A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission. | ||
PMC Biophys Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling. |
