GNE Polyclonal antibody

GNE Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 25079-1-AP

Synonyms

DMRV, GLCNE, GNE, IBM2, ManAc kinase, NM, Uae1, UDP GlcNAc 2 epimerase

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Validation Data Gallery

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  WB analysis using 25079-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25079-1-AP (GNE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25079-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25079-1-AP (GNE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25079-1-AP

  IP result of anti-GNE (IP:25079-1-AP, 4ug; Detection:25079-1-AP 1:500) with Jurkat cells lysate 3600ug.

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  IHC staining of human kidney using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, Jurkat cells, rat liver tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human kidney tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

25079-1-AP targets GNE in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNE fusion protein Ag18689
• Full Name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• Calculated Molecular Weight: 722 aa, 79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC121179
• Gene Symbol: GNE
• Gene ID (NCBI): 10020
• RRID: AB_2879887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Neoplasma; miR-550a-5p promotes the proliferation and migration of hepatocellular carcinoma by targeting GNE via the Wnt/β-catenin signaling pathway; Authors - Chun-Ming Li
human	WB	Cell Rep; Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins.; Authors - Joseph Hiatt
mouse	WB,IF,IP	Front Genome Ed; In vivo and in vitro genome editing to explore GNE functions; Authors - Nili Ilouz ; KO Validated
human	IHC	Cells; Loss of GNE Predicts Lymph Node Metastasis in Early Gastric Cancer; Authors - Xinying Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

Works well @ 1:50 for IHC on FFPE prostate tissue and 1:2000 for WB on LNCaP cells

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate