GNE Monoclonal antibody

GNE Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2A11E10

Cat no : 67649-1-Ig

Synonyms

DMRV, GLCNE, GNE, IBM2, ManAc kinase, NM, Uae1, UDP GlcNAc 2 epimerase

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Validation Data Gallery

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  WB analysis using 67649-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67649-1-Ig (GNE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67649-1-Ig targets GNE in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNE fusion protein Ag18702
• Full Name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• Calculated Molecular Weight: 722 aa, 79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC121179
• Gene Symbol: GNE
• Gene ID (NCBI): 10020
• RRID: AB_2882849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Neurol Sci; Clinical, genetic, and pathological characterization of GNE myopathy in China.; Authors - Xiao-Qing Lv