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GNL3 Polyclonal antibody

GNL3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15060-1-AP

Synonyms

C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells, HepG2 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15060-1-AP targets GNL3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNL3 fusion protein Ag7056
Full Name guanine nucleotide binding protein-like 3 (nucleolar)
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC001024
Gene Symbol GNL3
Gene ID (NCBI) 26354
RRIDAB_2263303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GNL3 antibody 15060-1-APDownload protocol
IHC protocol for GNL3 antibody 15060-1-APDownload protocol
IF protocol for GNL3 antibody 15060-1-APDownload protocol
IP protocol for GNL3 antibody 15060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Nanobiotechnology

Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer.

Authors - Yiran Zhang
humanWB

Cancer Manag Res

The oncogenic role of GNL3 in the progression and metastasis of osteosarcoma.

Authors - Tianyou Li
  • KD Validated