GNL3 Polyclonal antibody

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  WB analysis using 15060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15060-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15060-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15060-1-AP

  IP result of anti-GNL3 (IP:15060-1-AP, 4ug; Detection:15060-1-AP 1:600) with HeLa cells lysate 4000ug.

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  IHC staining of human oesophagus cancer using 15060-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 15060-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15060-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15060-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15060-1-AP (GNL3 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

GNL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15060-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NNP47, Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, E2IG3, E2 induced gene 3 protein

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, HeLa cells, HepG2 cells, Raji cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human oesophagus cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

15060-1-AP targets GNL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7056

  Product name: Recombinant human GNL3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 201-549 aa of BC001024

  Sequence: TVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV

• Full Name: guanine nucleotide binding protein-like 3 (nucleolar)
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC001024
• Gene Symbol: GNL3
• Gene ID (NCBI): 26354
• RRID: AB_2263303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BVP2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Nanobiotechnology; Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer.; Authors - Yiran Zhang
human	WB	Cancer Manag Res; The oncogenic role of GNL3 in the progression and metastasis of osteosarcoma.; Authors - Tianyou Li ; KD Validated