Tested Applications
| Positive WB detected in | rat kidney tissue, mouse kidney tissue |
| Positive IHC detected in | human breast cancer tissue, human spleen tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17105-1-AP targets GNPDA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10780 Product name: Recombinant human GNPDA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC015532 Sequence: MRLVILDNYDLASEWAAKYICNRIIQFKPGQDRYFTLGLPTGSTPLGCYKKLIEYHKNGHLSFKYVKTFNMDEYVGLPRNHPESYHSYMWNNFFKHIDIDPNNAHILDGNAADLQAECDAFENKIKEAGGIDLFVGGIGPDGHIAFNEPGSSLVSRTRLKTLAMDTILANAKYFDGDLSKVSTMALTVGVGTVMDAREVMILITGAHKAFALYKAIEGVNHMWTVSAFQQHPRTIFVCDEDATLELRVKTVKYFKGLMHVHNKLVDPLFSMKDGN Predict reactive species |
| Full Name | glucosamine-6-phosphate deaminase 2 |
| Calculated Molecular Weight | 275 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC015532 |
| Gene Symbol | GNPDA2 |
| Gene ID (NCBI) | 132789 |
| RRID | AB_2878350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GNPDA2(Glucosamine-6-phosphate deaminase 2) is also named as GNP2 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Human GNPDA1 and GNPDA2 shared the same fold and were predicted to form hexameric particles(PMID:12965206). This protein has 5 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 29 kDa, 23 kDa and 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GNPDA2 antibody 17105-1-AP | Download protocol |
| WB protocol for GNPDA2 antibody 17105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GNPDA2 Polyclonal antibody (Cat# 17105-1-AP) has a total of 2 citations. These appear in Glycobiology (2016) and International Journal of Endocrinology (2019). The research fields covered include hexosamine biosynthesis in keratinocytes, UDP-GlcNAc maintenance, hyaluronan synthesis, and adipogenesis in human adipose-derived mesenchymal stem cells. Both citations used the antibody for Western blot applications, with one study involving RNAi validation in human samples and the other in mouse samples.
| Species | Application | Title |
|---|---|---|
Glycobiology Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.
| ||
Int J Endocrinol GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells. |
