GNRHR2 Polyclonal antibody

GNRHR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20728-1-AP

Synonyms

GnRH II R, GNRHR2

---

Validation Data Gallery

• 

  WB analysis of human brain using 20728-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20728-1-AP (GNRHR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 20728-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20728-1-AP (GNRHR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 20728-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 20728-1-AP (GNRHR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20728-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 20728-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20728-1-AP (GNRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20728-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20728-1-AP (GNRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20728-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 20728-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 20728-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

• 

  IF Staining of HEK-293 using 20728-1-AP

  Immunofluorescent analysis of HEK-293 cells using 20728-1-AP (GNRHR2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Tested Applications

• Positive WB detected in: human brain tissue, human placenta tissue, human stomach tissue
• Positive IHC detected in: human kidney tissue, human liver cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20728-1-AP targets GNRHR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: gonadotropin-releasing hormone (type 2) receptor 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: NR_002328
• Gene Symbol: GNRHR2
• Gene ID (NCBI): 114814
• RRID: AB_2878728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.