GNS Polyclonal antibody

GNS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13044-1-AP

Synonyms

N-acetylglucosamine-6-sulfatase, Glucosamine-6-sulfatase, G6S, EC:3.1.6.14

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 13044-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13044-1-AP (GNS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13044-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13044-1-AP (GNS antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13044-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13044-1-AP (GNS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13044-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13044-1-AP (GNS antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of PC-3 using 13044-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13044-1-AP (GNS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3200
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13044-1-AP targets GNS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNS fusion protein Ag3712
• Full Name: glucosamine (N-acetyl)-6-sulfatase
• Calculated Molecular Weight: 552 aa, 62 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC012482
• Gene Symbol: GNS
• Gene ID (NCBI): 2799
• RRID: AB_2115308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Lysosomal protease deficiency or substrate overload induces an oxidative-stress mediated STAT3-dependent pathway of lysosomal homeostasis.; Authors - Jonathan Martínez-Fábregas