GOLGA3 Polyclonal antibody

GOLGA3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 21193-1-AP

Synonyms

MEA2, MEA 2, Golgin-160, Golgin 160, Golgi complex-associated protein of 170 kDa

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Validation Data Gallery

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  WB analysis of L02 using 21193-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21193-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21193-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21193-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21193-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21193-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21193-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOLGA3 antibody (21193-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, HEK-293 cells, HeLa cells, human heart tissue, human testis tissue, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IF: See 2 publications below

Product Information

21193-1-AP targets GOLGA3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GOLGA3 fusion protein Ag15531
• Full Name: golgi autoantigen, golgin subfamily a, 3
• Calculated Molecular Weight: 1498 aa, 167 kDa
• Observed Molecular Weight: 167 kDa
• GenBank Accession Number: BC142658
• Gene Symbol: GOLGA3
• Gene ID (NCBI): 2802
• RRID: AB_10733640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.; Authors - Gunjan Joshi
human	WB	PLoS Biol; A  conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.; Authors - Weichao Zhang
human	WB	J Biol Chem; GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.; Authors - Erpan Ahat
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human,mouse	WB,IF	PeerJ; Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis; Authors - Changtong Xu
	WB	Methods Mol Biol; Common Markers and Small Molecule Inhibitors in Golgi Studies; Authors - Sarah Bui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-02-2023)

Very good for WB.

• Applications: Western Blot