Tested Applications
| Positive WB detected in | HEK-293 cells, mouse lung tissue, HeLa cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17798-1-AP targets GORAB in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12002 Product name: Recombinant human GORAB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-246 aa of BC064945 Sequence: MSWAAVLAVAAARFGHFWGCRWPGPMAQGWAGFSEEELRRLKQTKDPFEPQRRLPAKKSRQQLQREKALVEQSQKLGLQDGSTSLLPEQLLSAPKQRVNVQKPPFSSPTLPSHFTLTSPVGDGQPQGIESQPKELGLENSHDGHNNVEILPPKPDCKLEKKKVELQEKSRWEVLQQEQRLMEEKNKRKKALLAKAIAERSKRTQAETMKLKRIQKELQALDDMVSADIGILRNRIDQASLDYSYAR Predict reactive species |
| Full Name | golgin, RAB6-interacting |
| Calculated Molecular Weight | 264aa,28 kDa; 394aa,45 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC064945 |
| Gene Symbol | GORAB |
| Gene ID (NCBI) | 92344 |
| RRID | AB_10755145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T7V8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GORAB, also known as Scyl1BP1, is a protein that localizes to the trans-side of the Golgi apparatus and plays a significant role in intracellular trafficking and protein glycosylation. It is composed of a central coiled-coil region that is responsible for Golgi targeting, likely through interactions with small GTPases Rab6 and Arf5. GORAB is considered a member of the golgin family of coiled-coil Golgi proteins, which are involved in vesicle tethering. GORAB has been implicated in several cellular functions. It has been proposed to function as a transcriptional activator for neurite outgrowth, as a modulator of MDM2 ubiquitylation impacting p53 levels and apoptosis, and has been shown to play a role in centriole duplication and ciliogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GORAB antibody 17798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GORAB Polyclonal antibody (Cat# 17798-1-AP) has 6 total citations. These appear in J Invest Dermatol, FASEB J, Cell Stress Chaperones, J Cell Sci, Mol Vis, and Methods Mol Biol. The research fields span Hedgehog signaling in hair follicle morphogenesis, embryonic lung maturation, replicative senescence and heat shock response, Golgi complex structural biology, Lowe oculocerebrorenal syndrome genetics, and Golgi apparatus immunofluorescence methodology.
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Gorab Is Required for Dermal Condensate Cells to Respond to Hedgehog Signals during Hair Follicle Morphogenesis.
| ||
FASEB J GORAB promotes embryonic lung maturation through antagonizing AKT phosphorylation, versican expression, and mesenchymal cell migration.
| ||
Cell Stress Chaperones Proteomic and phenotypic profiling of replicative-senescent human foreskin fibroblasts under brief heat shock. | ||
J Cell Sci Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex | ||
Mol Vis Identification and functional analysis of a novel oculocerebrorenal syndrome of Lowe (OCRL) gene variant in two pedigrees with varying phenotypes including isolated congenital cataract. | ||
