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  • KD/KO Validated

GORASP2 Polyclonal antibody

GORASP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 10598-1-AP


Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

Tested Applications

Positive WB detected inCOLO 320 cells, rat testis tissue, HeLa cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10598-1-AP targets GORASP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GORASP2 fusion protein Ag0950
Full Name golgi reassembly stacking protein 2, 55kDa
Calculated molecular weight 55 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC007770
Gene symbol GORASP2
Gene ID (NCBI) 26003
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.


Product Specific Protocols
WB protocol for GORASP2 antibody 10598-1-APDownload protocol
IHC protocol for GORASP2 antibody 10598-1-APDownload protocol
IF protocol for GORASP2 antibody 10598-1-APDownload protocol
IP protocol for GORASP2 antibody 10598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Dev Cell

A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport.

Authors - Mafalda Lopes-da-Silva

Int J Mol Sci

Mutations in GDAP1 Influence Structure and Function of the Trans-Golgi Network.

Authors - Katarzyna Binięda

Int J Mol Sci

Knock-Out of ACBD3 Leads to Dispersed Golgi Structure, but Unaffected Mitochondrial Functions in HEK293 and HeLa Cells.

Authors - Tereza Daňhelovská

J Exp Clin Cancer Res

Autophagy-based unconventional secretion of HMGB1 in glioblastoma promotes chemosensitivity to temozolomide through macrophage M1-like polarization.

Authors - Zhuang Li
  • KD Validated


SARS-CoV-2 triggers Golgi fragmentation via down-regulation of GRASP55 to facilitate viral trafficking.

Authors - Jianchao Zhang

Biol Open

GRASP55 restricts early-stage autophagy and regulates spatial organization of the early secretory network.

Authors - Jennifer Y Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hai (Verified Customer) (07-09-2021)

I used 1:400 dilution in 2%BSA/PBS and got strong signals. The cells in the image I uploaded was stained with mouse-Tubulin- AF633 and rabbit-GORASP2- AF488 and DAPI.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Melanoma cells
GORASP2 Antibody Immunofluorescence validation (1:400 dilution) in Melanoma cells (Cat no:10598-1-AP)

Ana (Verified Customer) (03-14-2021)

GORASP, golgi labeling in human primary fibroblasts. PFA 4% fixed cells. 1:100 primary ab in 5% GS PBST (0.1% triton) O/N 4ºC incubation. 1:500 secondary ab Alexafluor 488 1h RT.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Primary Fibroblasts
GORASP2 Antibody Immunofluorescence validation (1:100 dilution) in Human Primary Fibroblasts (Cat no:10598-1-AP)

Uxoa (Verified Customer) (02-18-2020)

GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Primary human fibroblasts
GORASP2 Antibody Immunofluorescence, validation (1:100 dilution) in Primary human fibroblasts (Cat no:10598-1-AP)