|Positive WB detected in||COLO 320 cells, rat testis tissue, HeLa cells, Raji cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10598-1-AP targets GORASP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GORASP2 fusion protein Ag0950|
|Full Name||golgi reassembly stacking protein 2, 55kDa|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC007770|
|Gene ID (NCBI)||26003|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
|Product Specific Protocols|
|WB protocol for GORASP2 antibody 10598-1-AP||Download protocol|
|IHC protocol for GORASP2 antibody 10598-1-AP||Download protocol|
|IF protocol for GORASP2 antibody 10598-1-AP||Download protocol|
|IP protocol for GORASP2 antibody 10598-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport.
Int J Mol Sci
Mutations in GDAP1 Influence Structure and Function of the Trans-Golgi Network.
Int J Mol Sci
Knock-Out of ACBD3 Leads to Dispersed Golgi Structure, but Unaffected Mitochondrial Functions in HEK293 and HeLa Cells.
GRASP55 restricts early-stage autophagy and regulates spatial organization of the early secretory network.
Biochem Biophys Res Commun
CD83 and GRASP55 interact in human dendritic cells.
DRAM1 deficiency affects the organization and function of the Golgi apparatus.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hai (Verified Customer) (07-09-2021)
I used 1:400 dilution in 2%BSA/PBS and got strong signals. The cells in the image I uploaded was stained with mouse-Tubulin- AF633 and rabbit-GORASP2- AF488 and DAPI.
Ana (Verified Customer) (03-14-2021)
GORASP, golgi labeling in human primary fibroblasts. PFA 4% fixed cells. 1:100 primary ab in 5% GS PBST (0.1% triton) O/N 4ºC incubation. 1:500 secondary ab Alexafluor 488 1h RT.
Uxoa (Verified Customer) (02-18-2020)
GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.