GORASP2 Recombinant antibody

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  WB analysis using 85077-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85077-5-RR (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 85077-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85077-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 85077-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 85077-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85077-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85077-5-RR against Human GORASP2 were performed. The affinity constant is 5.33 nM.

GORASP2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85077-5-RR

Clone No.242700C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2, Golgi phosphoprotein 6

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, Raji cells, COLO 320 cells, MDA-MB-453 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85077-5-RR targets GORASP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GORASP2 fusion protein Ag0950
• Full Name: golgi reassembly stacking protein 2, 55kDa
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC007770
• Gene Symbol: GORASP2
• Gene ID (NCBI): 26003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H8Y8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.