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  • KD/KO Validated

GOT1 Polyclonal antibody

GOT1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14886-1-AP


AATC, CAT, GIG18, GOT1, Transaminase A

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, mouse brain, rat brain
Positive IP detected inmouse brain tissue
Positive IF detected inL02 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14886-1-AP targets GOT1 in WB, IP, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOT1 fusion protein Ag6671
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Calculated molecular weight 46 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC000498
Gene symbol GOT1
Gene ID (NCBI) 2805
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.


Product Specific Protocols
WB protocol for GOT1 antibody 14886-1-APDownload protocol
IF protocol for GOT1 antibody 14886-1-APDownload protocol
IP protocol for GOT1 antibody 14886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Authors - Yuanyuan Wang

Am J Respir Cell Mol Biol

Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Authors - Robert B Hamanaka
  • KD Validated

Cell Rep

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Authors - Alex J Bott

Cell Rep

PMID: 31801093

Authors - Benjamin D Stein

Cell Rep

HIF1α Suppresses Tumor Cell Proliferation through Inhibition of Aspartate Biosynthesis.

Authors - FlorindaMeléndez-Rodríguez

Mol Med Rep

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Authors - Maojun Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Daniel (Verified Customer) (04-29-2019)

Strong band of the expected size. Weaker non-specific bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HK2 and RCC4 (human renal cells)
GOT1 Antibody Western Blot, validation (1:1000 dilution) in HK2 and RCC4 (human renal cells) (Cat no:14886-1-AP)