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GOT1 Polyclonal antibody
GOT1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IF, ELISA
Cat no : 14886-1-AP
|Positive WB detected in||HepG2 cells, L02 cells, mouse brain, rat brain|
|Positive IP detected in||mouse brain tissue|
|Positive IF detected in||L02 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14886-1-AP targets GOT1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GOT1 fusion protein Ag6671|
|Full Name||glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||43-46 kDa|
|GenBank accession number||BC000498|
|Gene ID (NCBI)||2805|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.
|Product Specific Protocols|
|WB protocol for GOT1 antibody 14886-1-AP||Download protocol|
|IF protocol for GOT1 antibody 14886-1-AP||Download protocol|
|IP protocol for GOT1 antibody 14886-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.
J Exp Med
Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniel (Verified Customer) (04-29-2019)
Strong band of the expected size. Weaker non-specific bands.