Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, mouse brain, rat brain |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14886-1-AP targets GOT1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6671 Product name: Recombinant human GOT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-413 aa of BC000498 Sequence: MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ Predict reactive species |
| Full Name | glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 43-46 kDa |
| GenBank Accession Number | BC000498 |
| Gene Symbol | GOT1 |
| Gene ID (NCBI) | 2805 |
| RRID | AB_2113630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17174 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GOT1 antibody 14886-1-AP | Download protocol |
| IP protocol for GOT1 antibody 14886-1-AP | Download protocol |
| WB protocol for GOT1 antibody 14886-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GOT1 Polyclonal antibody (Cat# 14886-1-AP) has 59 citations published in journals including Cell, Nature Cell Biology, Nature Communications, Cell Metabolism, Cell Research, Molecular Cell, EMBO Journal, Journal of Clinical Investigation, and Cancer Research. Research fields span cancer metabolism, glutamine/aspartate metabolism, ferroptosis, mitochondrial biology, viral replication, neurodegeneration, liver disease, stroke, and immunology. Cited applications include WB, IF, IP, and CoIP across human, mouse, rat, and pig species.
| Species | Application | Title |
|---|---|---|
Cell Systematic discovery of mutation-directed neo-protein-protein interactions in cancer. | ||
Cell Metab Compartmentalized branched-chain amino acid metabolism orchestrates colorectal cancer dissemination via an UMP-vimentin axis. | ||
Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. | ||
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Cell Res Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
| ||
Nat Cell Biol De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence
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Reviews
What customers say
The single review for this product reports use in Western Blot on human renal cells (HK2 and RCC4) at a 1:1000 dilution. The reviewer observed a strong band at the expected molecular weight, along with some weaker non-specific bands, and rated the product 4 out of 5. Overall, the feedback is positive, indicating reliable detection of the target with minor background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniel (Verified Customer) (04-29-2019) | Strong band of the expected size. Weaker non-specific bands.
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