GOT1 Monoclonal antibody, PBS Only

GOT1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, chicken

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A12E10

Cat no : 60317-1-PBS

Synonyms

CAT, GIG18, GOT1, Transaminase A

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Validation Data Gallery

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  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 60317-1-Ig (same clone as 60317-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOT1 antibody (60317-1-Ig, Clone: 5A12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

  1X10^6 HepG2 cells were stained with 0.20ug GOT1 antibody (60317-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60317-1-PBS targets GOT1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, chicken samples.

• Tested Reactivity: human, mouse, rat, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GOT1 fusion protein Ag7012
• Full Name: glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 40-46 kDa
• GenBank Accession Number: BC000498
• Gene Symbol: GOT1
• Gene ID (NCBI): 2805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.