GP repeat Polyclonal antibody

GP repeat Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24494-1-AP

Synonyms

C9RAN, GGGCC, GP repeat, GPGPGPGP, poly GP, poly PG



Tested Applications

Positive WB detected inRecombinant protein
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:500
Sample-dependent, check data in validation data gallery

Product Information

24494-1-AP targets GP repeat in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GP repeat
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GP repeated sequence. The recombinant protein 6*His tag-SMARCA4 or GST tag-SMARCA4 which contains the sequence "GPGPGPGPG" were used to detect this antibody.

Protocols

Product Specific Protocols
WB protocol for GP repeat antibody 24494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Neuropathol Appl Neurobiol

Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.

Authors - Yvonne Davidson
humanIHC

JCI Insight

Identification and therapeutic rescue of autophagosome and glutamate receptor defects in C9ORF72 and sporadic ALS neurons.

Authors - Yingxiao Shi
humanWB

Cancer Manag Res

Connexin 32 downregulation is critical for chemoresistance in oxaliplatin-resistant HCC cells associated with EMT.

Authors - Yan Yang
humanWB,IF

Int J Biochem Cell Biol

MEL-pep, an analog of melittin, disrupts cell membranes and reverses 5-fluorouracil resistance in human hepatocellular carcinoma cells.

Authors - Mengyun Ke
humanWB

J Cell Sci

SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.

Authors - Mirjana Malnar
humanWB

Glia

Mutant C9orf72 human iPSC-derived astrocytes cause non-cell autonomous motor neuron pathophysiology.

Authors - Chen Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Barbara (Verified Customer) (01-14-2019)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: lysates of transgenic flies expressing 36 repeats of GGGGCC