CD42b Polyclonal antibody

CD42b Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12860-1-AP

Synonyms

BSS, CD42b, CD42b alpha, GP1B, GP1BA



Tested Applications

Positive WB detected inHuman peripheral blood platelets, pig peripheral blood leukocyte, human blood
Positive IHC detected inhuman spleen tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12860-1-AP targets CD42b in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD42b fusion protein Ag3893
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated Molecular Weight 626 aa, 69 kDa
Observed Molecular Weight 145 kDa
GenBank Accession NumberBC027955
Gene Symbol CD42b
Gene ID (NCBI) 2811
RRIDAB_10644481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

Protocols

Product Specific Protocols
WB protocol for CD42b antibody 12860-1-APDownload protocol
IHC protocol for CD42b antibody 12860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Healthc Mater

Platelet Membrane-Fused Circulating Extracellular Vesicles Protect the Heart from Ischemia/Reperfusion Injury

Authors - Jizong Jiang
mouseIF

Cancer Lett

Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma

Authors - Qianyu Shi
ratWB

Theranostics

A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.

Authors - Peipei Jin
humanWB

Int J Biol Macromol

Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing

Authors - Hang Dong
mouseWB

ACS Biomater Sci Eng

Biomimetic Platform Based on Mesoporous Platinum for Multisynergistic Cancer Therapy.

Authors - Gaoqian Zhao
mouseIHC

Biochem Pharmacol

GSK669, a NOD2 receptor antagonist, inhibits thrombosis and oxidative stress via targeting platelet GPVI.

Authors - Guanxing Pan