CD42a / GP9 Monoclonal antibody

• 

  WB analysis of human peripheral blood platelets using 67271-1-Ig

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 67271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 67271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD42a / GP9 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67271-1-Ig

Clone No.2D4H1

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

---

Tested Applications

• Positive WB detected in: Human peripheral blood platelets tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67271-1-Ig targets CD42a / GP9 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6132

  Product name: Recombinant human GP9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-154 aa of BC030229

  Sequence: MPAWGALFLLWATAEATKDCPSPCTCRALETMGLWVDCRGHGLTALPALPARTRHLLLANNSLQSVPPGAFDHLPQLQTLDVTQNPWHCDCSLTYLRLWLEDRTPEALLQVRCASPSLAAHGPLGRLTGYQLGSCGWQLQASWVRPGVLWDVAL

• Full Name: glycoprotein IX (platelet)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC030229
• Gene Symbol: CD42a
• Gene ID (NCBI): 2815
• RRID: AB_2882540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14770
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.