GPAA1 Polyclonal antibody

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• GPAA1 Polyclonal antibody (10104-1-AP)

GPAA1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10104-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP-MS, ELISA

Synonyms

GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

• 

  WB analysis using 10104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 10104-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 10104-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HuH-7 cells, HepG2 cells, mouse brain tissue, rat brain tissue, MCF-7 cells, MKN-45 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10104-1-AP targets GPAA1 in WB, IHC, IF, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0152

  Product name: Recombinant human GPAA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 62-321 aa of BC004129

  Sequence: EQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRG

• Full Name: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 60-68 kDa
• GenBank Accession Number: BC004129
• Gene Symbol: GPAA1
• Gene ID (NCBI): 8733
• RRID: AB_2263708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43292
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.

Publications

What published studies show

The anti-GPAA1 antibody (Cat# 10104-1-AP) has 9 total citations published in journals including Cancer Research, Nature Communications, Cell Reports, Modern Pathology, International Immunopharmacology, Liver Research, Frontiers in Oncology, Life Science Alliance, and American Journal of Cancer Research. Research fields span cancer immunology (immune evasion, checkpoint biology), GPI-anchored membrane trafficking, viral entry mechanisms, inflammatory signaling, and protein degradation pathways.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP-MS	Cancer Res; Radiation-Enhanced CD24 Membrane Trafficking via GPI Anchoring Mediates Antitumor Immune Evasion.; Authors - Lingyi Kong ; KD Validated
mouse	IF	Nat Commun; CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection; Authors - B Vijayalakshmi Ayyar
human	WB	Cell Rep; Targeting the GPI transamidase subunit GPAA1 abrogates the CD24 immune checkpoint in ovarian cancer; Authors - Alok K Mishra ; KD Validated
human	IHC	Mod Pathol; Profiling the expression pattern of GPI transamidase complex subunits in human cancer.; Authors - Nagpal Jatin K JK
		Int Immunopharmacol; Vitamin D against diabetic adipose tissue inflammation through SHP-1/STAT3 pathway; Authors - Xiaonuo Wei
human	WB,IHC	Liver Res; Single-cell and machine learning approaches uncover intrinsic immune-evasion genes in the prognosis of hepatocellular carcinoma; Authors - Jiani Wang