Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells |
| Positive IHC detected in | human small intestine tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21622-1-AP targets GPBP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14877 Product name: Recombinant human GPBP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 293-473 aa of BC000267 Sequence: MRTDKKSEFLKALKRDRVEEEHEDESRAGSEKDDDSFNLHNSNSTHQERDINRNFDENEIPQENGNASVISQQIIRSSTFPQTDVLSSSLEAEHRLLKEMGWQEDSENDETCAPLTEDEMREFQVISEQLQKNGLRKNGILKNGLICDFKFGPWKNSTFKPTTENDDTETSSSDTSDDDDV Predict reactive species |
| Full Name | GC-rich promoter binding protein 1 |
| Calculated Molecular Weight | 473 aa, 53 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC000267 |
| Gene Symbol | GPBP1 |
| Gene ID (NCBI) | 65056 |
| RRID | AB_10732822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86WP2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPBP1 antibody 21622-1-AP | Download protocol |
| WB protocol for GPBP1 antibody 21622-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPBP1 Polyclonal antibody (Cat# 21622-1-AP) has 1 citation to date. It appears in Experimental & Molecular Medicine (2022), a study investigating how loss of RTN3 phenocopies chronic kidney disease through activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells. The research falls within the fields of renal biology and signaling pathway regulation. The antibody was used for Western blotting and immunoprecipitation in mouse samples.
| Species | Application | Title |
|---|---|---|
Exp Mol Med Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.
|
Reviews
What customers say
One reviewer (Joseph S., April 2025) rated this antibody 4/5 stars for Western Blot at 1:1500 dilution on HCT116 and HEK293 cells. The antibody was validated using GPBP1 knockout cells and detected two bands around 70 kDa, though some non-specific bands were observed. The reviewer recommended 5% BSA in PBS with 0.1% Tween 20 for blocking, noting that 5% milk caused high background. A protein loading of 15–40 μg per well was used with a 1-hour incubation at room temperature.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joseph (Verified Customer) (04-30-2025) | Protein amount used for detection: 15–40 μg total protein per well Specificity: Validated with GPBP1 KO cells; some non-specific bands observed Molecular weight: Detects two bands around 70 kDa Blocking: 5% BSA in PBS + 0.1% Tween 20 (5% milk caused high background) Primary antibody: 1:1500, 1 h at RT Wash: 3–4 × 5 min with PBS + 0.1% Tween 20
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