- Featured Product
- KD/KO Validated
Glypican 4 Polyclonal antibody
Glypican 4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 13048-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
Product Information
13048-1-AP targets Glypican 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glypican 4 fusion protein Ag3670 |
| Full Name | glypican 4 |
| Calculated Molecular Weight | 556 aa, 62 kDa |
| Observed Molecular Weight | 62 kDa |
| GenBank Accession Number | BC017166 |
| Gene Symbol | Glypican 4 |
| Gene ID (NCBI) | 2239 |
| RRID | AB_10640157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation (PMID: 9931407). In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus (PMID: 10585884). They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Human GPC4 is a 556-amino acid protein, and shows 93.5% identity to mouse K-glypican. The gene of GPC4 has been mapped to Xq26, and its mRNA is widely expressed in human tissues, including lung, kidney, heart, placenta, skeletal muscle, and pancreas. GPC4 has also been shown to be present in astrocytes, haematopoietic-progenitor and bone-marrow-stromal cells. Studies suggest that GPC4 is involved in the regulation of Wnt signaling and astrocyte GPC4 plays a role in formation of excitatory synapses (PMID: 22302992; 22722203). This antobody can recognize both full length (65 kDa) and cleaved (35 kDa) GPC4 as well as GPC6 (PMID: 18055828; 22722203).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Glypican 4 antibody 13048-1-AP | Download protocol |
| IHC protocol for Glypican 4 antibody 13048-1-AP | Download protocol |
| IF protocol for Glypican 4 antibody 13048-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors.
| ||
Neuron Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation. | ||
Sci Adv Cocaine preference and neuroadaptations are maintained by astrocytic NMDA receptors in the nucleus accumbens. | ||
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.
| ||
Nat Commun CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rebecca (Verified Customer) (02-21-2022) | Western performed on Biorad MiniPROTEAN 4-15% TGX precast gels and Precision Plus All Blue Protein Ladder and Non-boil reducing buffer. Blocking performed for 1h at room temperature using 5%BLOTTO. Primary antibody dilution was performed overnight at 4°C in 5%BLOTTO/0.05%Tween. Secondary antibody was LiCOR IRDye® 680RD Donkey anti-Mouse IgG (H + L) at 1:20000 dilution in PBS-Tween-SDS as per manufacturers instructions for 2h at room temperature. Blot showed a clear band of the expected size with very low-none background/off target binding in the membrane fraction. Non-specific bands were observed in the cytosolic fraction.
 |
FH Elena (Verified Customer) (11-19-2018) |
|
