GPD1 Polyclonal antibody

GPD1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13451-1-AP

Synonyms

cGPDH, GPD C, GPD1, GPDH C

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Validation Data Gallery

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  WB analysis using 13451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13451-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13451-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13451-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13451-1-AP (GPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, mouse heart tissue, rat liver tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13451-1-AP targets GPD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPD1 fusion protein Ag4278
• Full Name: glycerol-3-phosphate dehydrogenase 1 (soluble)
• Calculated Molecular Weight: 349 aa, 38 kDa
• Observed Molecular Weight: 32-42 kDa
• GenBank Accession Number: BC032234
• Gene Symbol: GPD1
• Gene ID (NCBI): 2819
• RRID: AB_10646471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Hematol Oncol; Allosteric activation of the metabolic enzyme GPD1 inhibits bladder cancer growth via the lysoPC-PAFR-TRPV2 axis.; Authors - Wenlong Zhang
human	WB	Proc Natl Acad Sci U S A; A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.; Authors - Shiqi Wu
rat	WB	Mol Biol Rep; Glucose enhances catecholamine-stimulated lipolysis via increased glycerol-3-phosphate synthesis in 3T3-L1 adipocytes and rat adipose tissue.; Authors - Nodoka Takeuchi
human	WB,IHC,IF	Cell Signal; Angiotensin II induces podocyte metabolic reprogramming from glycolysis to glycerol-3-phosphate biosynthesis; Authors - Zilv Luo
mouse	WB	Sci Rep; Identification of mouse soleus muscle proteins altered in response to changes in gravity loading; Authors - Yoko Ino
mouse	WB	Phytomedicine; Radix Astragali decoction improves liver regeneration by upregulating hepatic expression of aquaporin-9; Authors - Yanghao Li