GPD2 Polyclonal antibody

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• KD/KO Validated

GPD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17219-1-AP Copied!

Publications (25)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

EC:1.1.5.3, GDH2, Glycerol-3-phosphate dehydrogenase, mitochondrial, GPD 2, GPD M

• 

  WB analysis using 17219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17219-1-AP (GPD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse skeletal muscle using 17219-1-AP

  IP result of anti-GPD2 (IP:17219-1-AP, 4ug; Detection:17219-1-AP 1:8000) with mouse skeletal muscle tissue lysate 1280 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 17219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17219-1-AP (GPD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, mouse liver tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17219-1-AP targets GPD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11036

  Product name: Recombinant human GPD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 5-378 aa of BC019874

  Sequence: KAVKGTLVGGGALATVLGLSQFAHYRRKQMNLAYVKAADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIMLPVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVHVSGARCKDVLTGQEFDVRAKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIFFLPWQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEV

• Full Name: glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• Calculated Molecular Weight: 41 kDa, 81 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC019874
• Gene Symbol: GPD2
• Gene ID (NCBI): 2820
• RRID: AB_2112476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43304
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).

Publications

What published studies show

The anti-GPD2 antibody (Cat# 17219-1-AP) has 25 citations across journals including Cell Metabolism, Nature Immunology, Molecular Cell, PNAS, Journal of Biological Chemistry, and eLife. Research fields span mitochondrial metabolism, lipid biosynthesis, ferroptosis, gluconeogenesis, NAD⁺ homeostasis, cancer biology, and inflammatory responses, with applications primarily in Western blot across human, mouse, and rat species.

Automatically generated from the 25 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD+ to alleviate mitochondrial disease.; Authors - Shanshan Liu
mouse	WB	Nat Immunol; Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses.; Authors - P Kent Langston ; KO Validated
mouse,human	WB	Mol Cell; Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis; Authors - Cong-Hui Yao ; KD Validated
human	WB	Cell Death Dis; Targeting glycerophospholipid biosynthesis overcomes chemoresistance driven by SLFN11 loss in Ewing sarcoma.; Authors - Kasturee Chakraborty
human	WB	Proc Natl Acad Sci U S A; A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.; Authors - Shiqi Wu
		Int J Biol Macromol; Genistein and metformin regulate glycerol kinase and the enzymes of glycerol 3-phosphate shuttle in a differential manner in myocytes, hepatocytes and adipocytes; Authors - Superior Syngkli