GPD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 17219-1-AP

Synonyms

GDH2, GPD M, GPD2, GPDH M, GPDM, mGPDH, mtGPD

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Validation Data Gallery

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  WB analysis using 17219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17219-1-AP (GPD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 17219-1-AP

  IP result of anti-GPD2 (IP:17219-1-AP, 4ug; Detection:17219-1-AP 1:8000) with mouse skeletal muscle tissue lysate 1280 ug.

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  IHC staining of mouse testis using 17219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17219-1-AP (GPD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, mouse liver tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IP: See 1 publications below

Product Information

17219-1-AP targets GPD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPD2 fusion protein Ag11036
• Full Name: glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• Calculated Molecular Weight: 41 kDa, 81 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC019874
• Gene Symbol: GPD2
• Gene ID (NCBI): 2820
• RRID: AB_2112476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).

Publications

Species	Application	Title
mouse	WB	Nat Immunol; Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses.; Authors - P Kent Langston ; KO Validated
human	WB	Cell Metab; Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD+ to alleviate mitochondrial disease.; Authors - Shanshan Liu
human	WB	Proc Natl Acad Sci U S A; A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.; Authors - Shiqi Wu
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur
rat	WB	Free Radic Biol Med; Mitochondrial remodeling in the liver following chronic alcohol feeding to rats.; Authors - Derick Han
rat	WB	J Biol Chem; A refined analysis of superoxide production by mitochondrial sn-glycerol 3-phosphate dehydrogenase.; Authors - Orr Adam L AL