Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12681-1-AP targets Gephyrin/GPHN in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3370 Product name: Recombinant human GPHN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC030016 Sequence: MATEGMILTNHDHQIRVGVLTVSDSCFRNLAEDRSGINLKDLVQDPSLLGGTISAYKIVPDEIEEIKETLIDWCDEKELNLILTTGGTGFAPRDVTPEATKEVIEREAPGMALAMLMGSLNVTPLGMLSRPVCGIRGKTLIINLPGSKKGSQECFQFILPALPHAIDLLRDAIVKVKEVHDELEDLPSPPPPLSPPPTTSPHKQTEDKGVQCEEEEEEKKDSGVASTEDSSSSHITAAAIAAKKHPFYTSPAVVMAHGEQPIPGLINYSHHSTDERIPDSIISRGVQVLPRDTASLSTTPSESPRAQATSRLSTA Predict reactive species |
| Full Name | gephyrin |
| Calculated Molecular Weight | 769 aa, 83 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | BC030016 |
| Gene Symbol | GPHN |
| Gene ID (NCBI) | 10243 |
| RRID | AB_2247770 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Gephyrin/GPHN antibody 12681-1-AP | Download protocol |
| IP protocol for Gephyrin/GPHN antibody 12681-1-AP | Download protocol |
| WB protocol for Gephyrin/GPHN antibody 12681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Gephyrin/GPHN Polyclonal antibody (Cat# 12681-1-AP) has 20 citations spanning journals including Nature Neuroscience, Nature Communications, EMBO Reports, Theranostics, Cell Reports, PLoS Biology, Arthritis & Rheumatology, Journal of Neuroscience, and others. Research fields covered include epilepsy, autism, synaptic plasticity, microglial function, chronic pain, neurodegeneration (Alzheimer's, Alexander disease), GABA signaling, cardiac arrest–related neural repair, systemic sclerosis, and colon polyp pathology. Applications cited include WB, IF, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Nat Neurosci GABA-dependent microglial elimination of inhibitory synapses underlies neuronal hyperexcitability in epilepsy | ||
Adv Funct Mater Metabolically Glycoengineered Neural Stem Cells Boost Neural Repair After Cardiac Arrest | ||
Theranostics RPS23RG1 inhibits SORT1-mediated lysosomal degradation of MDGA2 to protect against autism | ||
Arthritis Rheumatol Anti-Gephyrin antibodies: A novel specificity in patients with systemic sclerosis and lower bowel dysfunction | ||
Environ Res 17β-Trenbolone modulates anxiety-related synaptic plasticity by affecting the interaction between hippocampal TACR3 and systemic testosterone in male mice | ||
Cell Rep Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars. One user performed immunofluorescence on human brain cortex paraffin sections at 1:100 dilution and reported clear staining, noting that the synaptic localization of gephyrin makes quantification in tissue somewhat challenging. The other user used it for immunohistochemistry on brain tissue at the same 1:100 dilution and simply confirmed it works fine. Overall, users find it reliable for brain tissue applications at a 1:100 dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (03-01-2024) | Gephyrin antibody (in red) works on human brain paraffin sections, although being found in the synapsis would make it difficult to quantify in the tissue.
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Deria (Verified Customer) (07-14-2022) | This antibody works fine
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