GPHN Monoclonal antibody

GPHN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D7C4

Cat no : 67995-1-Ig

Synonyms

Domain E, GEPH, gephyrin, GPH, GPHN, GPHRYN, KIAA1385, MPT adenylyltransferase, MPT Mo transferase

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Validation Data Gallery

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  WB analysis using 67995-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67995-1-Ig (GPHN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67995-1-Ig targets GPHN in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPHN fusion protein Ag23708
• Full Name: gephyrin
• Calculated Molecular Weight: 769 aa, 83 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC030016
• Gene Symbol: GPHN
• Gene ID (NCBI): 10243
• RRID: AB_2918744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.