GPI Monoclonal antibody

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• KD/KO Validated

GPI Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11E4

Cat no : 67178-1-Ig

Synonyms

Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9, AMF

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Validation Data Gallery

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  WB analysis using 67178-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67178-1-Ig (GPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67178-1-Ig

  WB result of GPI antibody (67178-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

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  WB analysis of HeLa using 67178-1-Ig

  WB result of GPI antibody (67178-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

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  IHC staining of human lung cancer using 67178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 67178-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GPI antibody (67178-1-Ig, Clone: 2F11E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, PC-3 cells, LNCaP cells, HEK-293 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67178-1-Ig targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPI fusion protein Ag7419
• Full Name: glucose phosphate isomerase
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC004982
• Gene Symbol: GPI
• Gene ID (NCBI): 2821
• RRID: AB_2882474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).