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GPI Monoclonal antibody

GPI Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1









Cat no : 67178-1-Ig


AMF, Autocrine motility factor, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, PC-3 cells, LNCaP cells, HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67178-1-Ig targets GPI in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPI fusion protein Ag7419
Full Name glucose phosphate isomerase
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 55-64 kDa
GenBank Accession NumberBC004982
Gene Symbol GPI
Gene ID (NCBI) 2821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).


Product Specific Protocols
WB protocol for GPI antibody 67178-1-IgDownload protocol
IHC protocol for GPI antibody 67178-1-IgDownload protocol
IF protocol for GPI antibody 67178-1-IgDownload protocol
Standard Protocols
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