GPM6A Polyclonal antibody

GPM6A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15044-1-AP

Synonyms

glycoprotein M6A, GPM6, GPM6A

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Validation Data Gallery

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  WB analysis using 15044-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15044-1-AP (GPM6A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15044-1-AP

  IP result of anti-GPM6A (IP:15044-1-AP, 3ug; Detection:15044-1-AP 1:800) with mouse brain tissue lysate 6500ug.

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  IHC staining of mouse brain using 15044-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15044-1-AP (GPM6A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

15044-1-AP targets GPM6A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPM6A fusion protein Ag6915
• Full Name: glycoprotein M6A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC022528
• Gene Symbol: GPM6A
• Gene ID (NCBI): 2823
• RRID: AB_2232574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycoprotein M6A (GPM6A, or M6A) is known as a multi-pass transmembrane protein and an abundant cell surface protein on neurons in the central nervous system (CNS). M6A belongs to the myelin proteolipid protein family and is involved in neuronal differentiation, including differentiation and migration of neuronal stem cell. M6A is identified as a retinal membrane protein that is strongly expressed at embryonic stages. In mouse adult retina, M6A plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation via regulation of MAPK and Src signaling pathways.

Publications

Species	Application	Title
human	WB,IF	Lab Invest; GPM6A expression is suppressed in hepatocellular carcinoma through miRNA-96 production.; Authors - Zong-Rui Li