GPR103 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR103 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25247-1-AP

Synonyms

AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155

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Validation Data Gallery

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  WB analysis of mouse brain using 25247-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25247-1-AP (GPR103 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 25247-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 25247-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 25247-1-AP is GPR103 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR103 fusion protein Ag19048
• Full Name: pyroglutamylated RFamide peptide receptor
• Calculated Molecular Weight: 431 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC128133
• Gene Symbol: GPR103
• Gene ID (NCBI): 84109
• RRID: AB_2879987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Neurochem; N-glycosylation of the human neuropeptide QRFP receptor (QRFPR) is essential for ligand binding and receptor activation.; Authors - Weiwei Wang
human	WB	J Clin Lab Anal; The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.; Authors - Huanzheng Li ; KD Validated