GPR111 Polyclonal antibody

GPR111 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24965-1-AP

Synonyms

G protein coupled receptor 111, GPR111, hGPCR35, PGR20

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Validation Data Gallery

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  IHC staining of human brain using 24965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24965-1-AP (GPR111 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 24965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24965-1-AP (GPR111 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cerebellum using 24965-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24965-1-AP (GPR111 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cerebellum using 24965-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24965-1-AP (GPR111 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human brain tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 24965-1-AP is GPR111 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR111 fusion protein Ag21705
• Full Name: G protein-coupled receptor 111
• Calculated Molecular Weight: 708 aa, 79 kDa
• GenBank Accession Number: BC157025
• Gene Symbol: GPR111
• Gene ID (NCBI): 222611
• RRID: AB_2879823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	FASEB J; Deficiency of G protein-coupled receptor Gpr111/Adgrf2 causes enamel hypomineralization in mice by alteration of the expression of kallikrein-related peptidase 4 (Klk4) during pH cycling process; Authors - Yuta Chiba