GPR125 Polyclonal antibody

GPR125 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11912-1-AP

Synonyms

G protein coupled receptor 125, GPR125, PGR21, TEM5L

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Validation Data Gallery

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  WB analysis of pig brain using 11912-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 11912-1-AP (GPR125 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11912-1-AP (GPR125 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11912-1-AP (GPR125 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11912-1-AP targets GPR125 in WB, IHC, ELISA applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR125 fusion protein Ag2513
• Full Name: G protein-coupled receptor 125
• Calculated Molecular Weight: 1321 aa, 146 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC026009
• Gene Symbol: GPR125
• Gene ID (NCBI): 166647
• RRID: AB_2877804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.