Tested Applications
| Positive IHC detected in | mouse kidney tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
17774-1-AP targets GPR126 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12195 Product name: Recombinant human GPR126 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 45-399 aa of BC075798 Sequence: LSNPSGTFTSPCYPNDYPNSQACMWTLRAPTGYIIQITFNDFDIEEAPNCIYDSLSLDNGESQTKFCGATAKGLSFNSSANEMHVSFSSDFSIQKKGFNASYIRVAVSLRNQKVILPQTSDAYQVSVAKSISIPELSAFTLCFEATKVGHEDSDWTAFSYSNASFTQLLSFGKAKSGYFLSISDSQCLLNNALPVKEKEDIFAESFEQLCLVWNNSLGSIGVNFKRNYETVPCDSTISKVIPGNGKLLLGSNQNEIVSLKGDIYNFRLWNFTMNAKILSNLSCNVKGNVVDWQNDFWNIPNLALKAESNLSCGSYLIPLPAAELASCADLGTLCQDGIIYRISVVIQNILRHPEV Predict reactive species |
| Full Name | G protein-coupled receptor 126 |
| Calculated Molecular Weight | 1222 aa, 137 kDa |
| GenBank Accession Number | BC075798 |
| Gene Symbol | GPR126 |
| Gene ID (NCBI) | 57211 |
| RRID | AB_2878439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86SQ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPR126 (G protein-coupled receptor 126) is a member of the adhesion GPCR family. GPR126 is all widely expressed on stromal cells (PMID: 25713288). It is essential for normal differentiation of promyelinating Schwann cells and for normal myelination of axons (PMID: 24227709). GPR126 regulates neural, cardiac and ear development by G-protein- and/or N-terminus-dependent signaling (PMID: 24082093, PMID: 24067352). GPR126 also stimulates VEGF signaling and angiogenesis by modulating VEGF receptor 2 expression through STAT5 and GATA2 in endothelial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR126 antibody 17774-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Progesterone activates GPR126 to promote breast cancer development via the Gi pathway.
| ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (10-04-2021) | calculated size is 130 kd. this antibody recognized major bands at 65, 55 kd.
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FH Fiona (Verified Customer) (01-09-2020) | Immunofluorescence staining on A549 cells to look at expression. Staining was clean with little background, however some some very bright artefacts.
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