GPR183/EBI2 Polyclonal antibody

• 

  WB analysis of Jurkat using 12377-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12377-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12377-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12377-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12377-1-AP

  IP result of anti-GPR183/EBI2 (IP:12377-1-AP, 4ug; Detection:12377-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 12377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR183/EBI2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12377-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

EBI2, GPR183, EBV-induced G-protein coupled receptor 2, Epstein-Barr virus-induced G-protein coupled receptor 2, G protein coupled receptor 183

---

Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, mouse lung tissue, Raji cells
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

12377-1-AP targets GPR183/EBI2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3035

  Product name: Recombinant human GPR183 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-361 aa of BC020752

  Sequence: MDIQMANNFTPPSATPQGNDCDLYAHHSTARIVMPLHYSLVFIIGLVGNLLALVVIVQNRKKINSTTLYSTNLVISDILFTTALPTRIAYYAMGFDWRIGDALCRITALVFYINTYAGVNFMTCLSIDRFIAVVHPLRYNKIKRIEHAKGVCIFVWILVFAQTLPLLINPMSKQEAERITCMEYPNFEETKSLPWILLGACFIGYVLPLIIILICYSQICCKLFRTAKQNPLTEKSGVNKKALNTIILIIVVFVLCFTPYHVAIIQHMIKKLRFSNFLECSQRHSFQISLHFTVCLMNFNCCMDPFIYFFACKGYKRKVMRMLKRQVSVSISSAVKSAPEENSREMTETQMMIHSKSSNGK

• Full Name: G protein-coupled receptor 183
• Calculated Molecular Weight: 361 aa, 41 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC020752
• Gene Symbol: EBI2
• Gene ID (NCBI): 1880
• RRID: AB_2114261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32249
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G-protein-coupled receptor 183, also known as Epstein-Barr virusinduced gene 2 (EBI2), was initially identified in B cells and is the most upregulated gene after infection with Epstein-Barr virus (PMID:37353188). GPR183 is known to play a key role in the development of antibody responses in secondary lymphoid organs (PMID:23473835).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Allergy Clin Immunol; Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression.; Authors - Hulse Kathryn E KE
human	WB	J Pharmacol Exp Ther; 7α,25-Dihydroxycholesterol Suppresses Hepatocellular Steatosis through GPR183/EBI2 in Mouse and Human Hepatocytes.; Authors - Jin Huang
mouse	WB	Neurotox Res; Phoenixin-20 Ameliorates Lipopolysaccharide-Induced Activation of Microglial NLRP3 Inflammasome.; Authors - Xiangliang Zeng
mouse	WB	Eur J Pharmacol; Activation of G-protein-coupled receptor 183 initiates inflammatory pain via macrophage CCL22 secretion; Authors - Zhenhua Qi
mouse	WB	Br J Nutr; RNA-seq screening and gene function analysis uncover GPR183 as a key mediator for methionine to stimulate milk synthesis in mouse mammary epithelial cells; Authors - Yuwen Zhou