GPR4 Polyclonal antibody

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  IHC staining of mouse lung using 28232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 28232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR4 Polyclonal Antibody for IHC, ELISA

Cat No. 28232-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

G protein coupled receptor 4, G-protein coupled receptor 4, G-protein coupled receptor 6C.l

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Tested Applications

• Positive IHC detected in: mouse lung tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28232-1-AP targets GPR4 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28273

  Product name: Recombinant human GPR4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 203-362 aa of BC067535

  Sequence: GILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

• Full Name: G protein-coupled receptor 4
• Calculated Molecular Weight: 362 aa, 41 kDa
• GenBank Accession Number: BC067535
• Gene Symbol: GPR4
• Gene ID (NCBI): 2828
• RRID: AB_3086039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46093
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR4 (G-protein-coupled receptor 4) is a proton-sensing G-protein-coupled receptor (GPCR) that contributes to neovascularization in acidic microenvironments (PMID: 33632325). GPR4 is highly expressed in vascular endothelial cells (ECs) and blood vessel-rich tissues such as the lung, kidney, heart, and liver. Moreover, GPR4 has been shown to mediate EC inflammatory responses to acidosis and is central for leukocyte-endothelium interaction (PMID: 27940273).