GPR50 Polyclonal antibody

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  WB analysis using 21514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21514-1-AP (GPR50 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21514-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21514-1-AP (GPR50 antibody) at dilution of 1:500.

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  IF Staining of SH-SY5Y cells using 21514-1-AP

  IF result of GPR50 antibody (21514-1-AP, 1:1,000) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.

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  IF Staining of mouse Hippocamus tissue using 21514-1-AP

  IF result of GPR50 antibody (21514-1-AP, 1:50) with mouse hippocampus by Dr. Qian Li, University of Edinburgh.

GPR50 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 21514-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

Melatonin related receptor, Mel1c, H9, G protein-coupled receptor 50, G protein coupled receptor 50

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: SH-SY5Y cells, mouse Hippocamus tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

21514-1-AP targets GPR50 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16040

  Product name: Recombinant human GPR50 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 245-617 aa of BC103696

  Sequence: LFAVCWCPINVLTVLVAVSPKEMAGKIPNWLYLAAYFIAYFNSCLNAVIYGLLNENFRREYWTIFHAMRHPIIFFSGLISDIREMQEARTLARARAHARDQAREQDRAHACPAVEETPMNVRNVPLPGDAAAGHPDRASGHPKPHSRSSSAYRKSASTHHKSVFSHSKAASGHLKPVSGHSKPASGHPKSATVYPKPASVHFKADSVHFKGDSVHFKPDSVHFKPASSNPKPITGHHVSAGSHSKSAFSAATSHPKPTTGHIKPATSHAEPTTADYPKPATTSHPKPTAADNPELSASHCPEIPAIAHPVSDDSDLPESASSPAAGPTKPAASQLESDTIADLPDPTVVTTSTNDYHDVVVVDVEDDPDEMAV

• Full Name: G protein-coupled receptor 50
• Calculated Molecular Weight: 617 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC103696
• Gene Symbol: GPR50
• Gene ID (NCBI): 9248
• RRID: AB_2878874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13585
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		PLoS Biol; A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.; Authors - Yingying Zhang
mouse	WB,CoIP	Cell Death Dis; GPR50 regulates neuronal development as a mitophagy receptor; Authors - Ji-Chuan Liu