GPR75 Polyclonal antibody

GPR75 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17972-1-AP

Synonyms

Probable G-protein coupled receptor 75

---

Validation Data Gallery

• 

  IHC staining of mouse brain using 17972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17972-1-AP (GPR75 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17972-1-AP (GPR75 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17972-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17972-1-AP (GPR75 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17972-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17972-1-AP (GPR75 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of rat brain using 17972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GPR75 antibody (17972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 17972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GPR75 antibody (17972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 17972-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using GPR75 antibody (17972-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IHC detected in: mouse brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17972-1-AP targets GPR75 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR75 fusion protein Ag12053
• Full Name: G protein-coupled receptor 75
• Calculated Molecular Weight: 540 aa, 59 kDa
• GenBank Accession Number: BC067475
• Gene Symbol: GPR75
• Gene ID (NCBI): 10936
• RRID: AB_2878475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.