GPRASP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPRASP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 12159-1-AP

Synonyms

GASP 2, GASP2, GPRASP2

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 12159-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12159-1-AP (GPRASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12159-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12159-1-AP (GPRASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12159-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12159-1-AP (GPRASP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12159-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12159-1-AP (GPRASP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

12159-1-AP targets GPRASP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPRASP2 fusion protein Ag2800
• Full Name: G protein-coupled receptor associated sorting protein 2
• Calculated Molecular Weight: 838 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC013576
• Gene Symbol: GPRASP2
• Gene ID (NCBI): 114928
• RRID: AB_2232653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice.; Authors - Mohamed Edfawy ; KO Validated
mouse	IHC	J Med Genet; GPRASP2, a novel causative gene mutated in an X-linked recessive syndromic hearing loss.; Authors - Guangqian Xing

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Mohamed (Verified Customer) (08-07-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse tissue and Hippocampal rat culture