GPSN2 Recombinant antibody, PBS Only

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  WB analysis using 83592-4-RR (same clone as 83592-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83592-4-RR (GPSN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83592-4-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 83592-4-RR (same clone as 83592-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using GPSN2 antibody (83592-4-RR, Clone: 240472D4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 83592-4-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 83592-4-RR (same clone as 83592-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using GPSN2 antibody (83592-4-RR, Clone: 240472D4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83592-4-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 83592-4-RR (same clone as 83592-4-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug GPSN2 Recombinant antibody (83592-4-RR, Clone:240472D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83592-4-PBS in a different storage buffer formulation.

GPSN2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83592-4-PBS

Clone No.240472D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

TER, TECR, Synaptic glycoprotein SC2, SC2, EC:1.3.1.93

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Product Information

83592-4-PBS targets GPSN2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPSN2 fusion protein Ag32598
• Full Name: glycoprotein, synaptic 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC002425
• Gene Symbol: GPSN2
• Gene ID (NCBI): 9524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NZ01
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Glycoprotein synaptic 2 (GPSN2), also known as TER and TECR, functions as a trans-2,3-enoyl-CoA reductase and reduces long and very long chain fatty acid trans-2,3-enoyl-CoA substrates (PMID: 12482854).