GPX4 Monoclonal antibody

• Featured Product
• KD/KO Validated

GPX4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

138

CloneNo.

3F5G5

Cat no : 67763-1-Ig

Synonyms

Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx

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Validation Data Gallery

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  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67763-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67763-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: EC109 cells, NCCIT cells, rabbit testis tissue, pig brain tissue, human platelet tissue, rat testis tisssue, mouse testis tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue, Hela cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, 4T1 cells, ATDC-5 cells
• Positive IHC detected in: human liver cancer tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 128 publications below
• IHC: See 28 publications below
• IF: See 23 publications below
• IP: See 1 publications below

Product Information

67763-1-Ig targets GPX4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Cited Reactivity: human, mouse, rat, chicken, goat, pig, sheep
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPX4 fusion protein Ag30650
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Observed molecular weight: 20-23 kDa
• GenBank accession number: BC021567
• Gene symbol: GPX4
• Gene ID (NCBI): 2879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Publications

Species	Application	Title
mouse	WB	Cell Host Microbe; Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice; Authors - Yunong Zeng
human	WB	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao
rat	WB	Redox Biol; SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway.; Authors - Wen-Yuan Wu
human,mouse	WB	Free Radic Biol Med; Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity; Authors - Bin Leng
human	WB	Oxid Med Cell Longev; Curcumin Induces Ferroptosis in Follicular Thyroid Cancer by Upregulating HO-1 Expression; Authors - Huanjie Chen
mouse	WB,IF	Sci Total Environ; Cadmium induces ferroptosis mediated inflammation by activating Gpx4/Ager/p65 axis in pancreatic β-cells; Authors - Huihui Hong