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GPX4 Monoclonal antibody
GPX4 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, rabbit, pig and More (4)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
138
CloneNo.
3F5G5
Cat no : 67763-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | EC109 cells, NCCIT cells, rabbit testis tissue, pig brain tissue, human platelet tissue, rat testis tisssue, mouse testis tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue, Hela cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, 4T1 cells, ATDC-5 cells |
Positive IHC detected in | human liver cancer tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 128 publications below |
IHC | See 28 publications below |
IF | See 23 publications below |
IP | See 1 publications below |
Product Information
67763-1-Ig targets GPX4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
Tested Reactivity | Human, mouse, rat, rabbit, pig |
Cited Reactivity | human, mouse, rat, chicken, goat, pig, sheep |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | GPX4 fusion protein Ag30650 |
Full Name | glutathione peroxidase 4 (phospholipid hydroperoxidase) |
Observed molecular weight | 20-23 kDa |
GenBank accession number | BC021567 |
Gene symbol | GPX4 |
Gene ID (NCBI) | 2879 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.
Protocols
Product Specific Protocols | |
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WB protocol for GPX4 antibody 67763-1-Ig | Download protocol |
IHC protocol for GPX4 antibody 67763-1-Ig | Download protocol |
IF protocol for GPX4 antibody 67763-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Host Microbe Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice | ||
ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells | ||
Redox Biol SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway. | ||
Free Radic Biol Med Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity | ||
Oxid Med Cell Longev Curcumin Induces Ferroptosis in Follicular Thyroid Cancer by Upregulating HO-1 Expression | ||
Sci Total Environ Cadmium induces ferroptosis mediated inflammation by activating Gpx4/Ager/p65 axis in pancreatic β-cells |