GPX5 Polyclonal antibody

GPX5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18731-1-AP

Synonyms

EGLP, Glutathione peroxidase 5, GPx 5, GPX5, GSHPx 5



Tested Applications

Positive WB detected inmouse testis tissue, PC-3 cells, human testis tissue, mouse liver tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18731-1-AP targets GPX5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, pig, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glutathione peroxidase 5 (epididymal androgen-related protein)
Calculated molecular weight 25 kDa
Observed molecular weight 22-28 kDa
GenBank accession numberNM_001509
Gene symbol GPX5
Gene ID (NCBI) 2880
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.

Protocols

Product Specific Protocols
WB protocol for GPX5 antibody 18731-1-APDownload protocol
IHC protocol for GPX5 antibody 18731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Andrology

Comparative analysis of boar seminal plasma proteome from different freezability ejaculates and identification of Fibronectin 1 as sperm freezability marker.

Authors - I Vilagran
humanWB

Anim Reprod Sci

Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality.

Authors - Ingrid Vilagran
swineWB

Theriogenology

Effects of kojic acid on boar sperm quality and anti-bacterial activity during liquid preservation at 17 C.

Authors - Weike Shaoyong
mouseIF

Sci Rep

Proteasome activators, PA28γ and PA200, play indispensable roles in male fertility.

Authors - Lin Huang