GPX5 Polyclonal antibody

GPX5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 18731-1-AP

Synonyms

EGLP, Glutathione peroxidase 5, GPx 5, GPX5, GSHPx 5

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Validation Data Gallery

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  WB analysis of mouse testis using 18731-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18731-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18731-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 18731-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18731-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 18731-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, PC-3 cells, human testis tissue, mouse liver tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IF: See 2 publications below

Product Information

18731-1-AP targets GPX5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glutathione peroxidase 5 (epididymal androgen-related protein)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 22-28 kDa
• GenBank Accession Number: NM_001509
• Gene Symbol: GPX5
• Gene ID (NCBI): 2880
• RRID: AB_10596913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.

Publications

Species	Application	Title
goat	WB	Oxid Med Cell Longev; Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.; Authors - Tian-Yu Feng
mouse	IF	Sci Rep; Proteasome activators, PA28γ and PA200, play indispensable roles in male fertility.; Authors - Lin Huang
mouse	WB	Mol Biol Rep; Autophagy-related 7 proteindependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell; Authors - Fanhong Lin
pig	WB	Animals (Basel); Effect of Boar Sperm Proteins and Quality Changes on Field Fertility.; Authors - Ilias Michos
pig	WB	Theriogenology; Effects of kojic acid on boar sperm quality and anti-bacterial activity during liquid preservation at 17 C.; Authors - Weike Shaoyong
human	WB	Anim Reprod Sci; Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality.; Authors - Ingrid Vilagran