Tested Applications
| Positive WB detected in | mouse testis tissue, PC-3 cells, human testis tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18731-1-AP targets GPX5 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | glutathione peroxidase 5 (epididymal androgen-related protein) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 22-28 kDa |
| GenBank Accession Number | NM_001509 |
| Gene Symbol | GPX5 |
| Gene ID (NCBI) | 2880 |
| RRID | AB_10596913 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75715 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPX5 antibody 18731-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPX5 Polyclonal Antibody (Cat# 18731-1-AP) has 15 citations published in journals including Antioxidants, Oxid Med Cell Longev, Reprod Biol Endocrinol, Int J Mol Sci, Exp Gerontol, Sci Rep, Genomics, Andrology, Mol Biol Rep, Animals, Theriogenology, Anim Reprod Sci, and Cryobiology. Research fields span reproductive biology (sperm quality, Leydig cell function, epididymal aging), oxidative stress and antioxidant signaling, mitochondrial dysfunction, autophagy, and proteomics across pig, mouse, goat, and human species.
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) Supplementing Boar Diet with Nicotinamide Mononucleotide Improves Sperm Quality Probably through the Activation of the SIRT3 Signaling Pathway | ||
Oxid Med Cell Longev Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway. | ||
Reprod Biol Endocrinol Melatonin ameliorates diabetic hyperglycaemia-induced impairment of Leydig cell steroidogenic function through activation of SIRT1 pathway | ||
Int J Mol Sci Development of a Normal Porcine Cell Line Growing in a Heme-Supplemented, Serum-Free Condition for Cultured Meat | ||
Exp Gerontol Contribution of impaired autophagy, mitochondrial dysfunction and abnormal lipolysis to epididymal aging in mice | ||
Sci Rep Proteasome activators, PA28γ and PA200, play indispensable roles in male fertility. |
Reviews
What customers say
The single review for 18731-1-AP is a 5-star rating from Mounika Vallakati (January 2026). The reviewer used the antibody at a 1:800 dilution for Western Blot, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation, describing the results as "bright bands" with "good illuminance" and overall calling it "good work." The lot number was 00070810.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (01-08-2026) | bright bands, good illuminance, Good work!!
|
