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  • KD/KO Validated

GPX7 Polyclonal antibody

GPX7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13501-1-AP

Synonyms

CL683, glutathione peroxidase 7, GPx 7, GPX6, GPX7, GSHPx 7



Tested Applications

Positive WB detected inhuman liver tissue, human placenta tissue, human brain tissue, Transfected HEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13501-1-AP targets GPX7 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPX7 fusion protein Ag4328
Full Name glutathione peroxidase 7
Calculated molecular weight 187 aa, 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC032788
Gene symbol GPX7
Gene ID (NCBI) 2882
RRIDAB_2112560
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330). This antibody can ve detected as 18-21kDa due to signal peptide splicing.

Protocols

Product Specific Protocols
WB protocol for GPX7 antibody 13501-1-APDownload protocol
IHC protocol for GPX7 antibody 13501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gut

Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells.

Authors - Peng Dunfa D
humanWB

Gut

Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma.

Authors - Peng Dunfa D
mouseWB

EMBO Mol Med

Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human.

Authors - Chang Yi-Cheng YC
humanIHC

Oncogene

Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

Authors - Qingcai Meng
humanWB

Carcinogenesis

Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesi.

Authors - Dun-Fa Peng
  • KD Validated
WB

Free Radic Biol Med

GPx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum.

Authors - Thomas Ramming