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  • KD/KO Validated

GPX7 Polyclonal antibody

GPX7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13501-1-AP

Synonyms

CL683, glutathione peroxidase 7, GPx 7, GPX6, GPX7, GSHPx 7



Tested Applications

Positive WB detected inhuman liver tissue, human placenta tissue, human brain tissue, Transfected HEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13501-1-AP targets GPX7 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPX7 fusion protein Ag4328
Full Name glutathione peroxidase 7
Calculated molecular weight 187 aa, 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC032788
Gene symbol GPX7
Gene ID (NCBI) 2882
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330). This antibody can ve detected as 18-21kDa due to signal peptide splicing.

Protocols

Product Specific Protocols
WB protocol for GPX7 antibody 13501-1-APDownload protocol
IHC protocol for GPX7 antibody 13501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Glutathione peroxidase 7 suppresses cancer cell growth and is hypermethylated in gastric cancer.

Authors - Zheng Chen
chickenIHC

Poult Sci

The development of antioxidant system in the intestinal tract of broiler chickens.

Authors - Dazhi Tang
humanWB

J Cancer

Glutathione Peroxidase 7 Suppresses Bile Salt-Induced Expression of Pro-Inflammatory Cytokines in Barrett's Carcinogenesis.

Authors - Dun-Fa Peng
humanWB

BMB Rep

GPx7 ameliorates non-alcoholic steatohepatitis by regulating oxidative stress.

Authors - Hyeon Ju Kim
human,mouseWB,IHC

J Cell Mol Med

GPX7 Facilitates BMSCs Osteoblastogenesis via ER Stress and mTOR Pathway.

Authors - Xuchen Hu
  • KD Validated
humanWB,IF

Front Oncol

GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma.

Authors - Yan Zhou
  • KD Validated