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GPX7 Polyclonal antibody

GPX7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13501-1-AP

Synonyms

CL683, glutathione peroxidase 7, GPx 7, GPX6, GPX7, GSHPx 7



Tested Applications

Positive WB detected inhuman liver tissue, human brain tissue, Transfected HEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13501-1-AP targets GPX7 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity broiler chick, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPX7 fusion protein Ag4328
Full Name glutathione peroxidase 7
Calculated molecular weight 187 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC032788
Gene symbol GPX7
Gene ID (NCBI) 2882
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330).

Protocols

Product Specific Protocols
WB protocol for GPX7 antibody 13501-1-APDownload protocol
IHC protocol for GPX7 antibody 13501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Glutathione peroxidase 7 suppresses cancer cell growth and is hypermethylated in gastric cancer.

Authors - Zheng Chen
broiler chickIHC

Poult Sci

The development of antioxidant system in the intestinal tract of broiler chickens.

Authors - Dazhi Tang
humanWB

J Cancer

Glutathione Peroxidase 7 Suppresses Bile Salt-Induced Expression of Pro-Inflammatory Cytokines in Barrett's Carcinogenesis.

Authors - Dun-Fa Peng
humanWB

BMB Rep

GPx7 ameliorates non-alcoholic steatohepatitis by regulating oxidative stress.

Authors - Hyeon Ju Kim
WB

Free Radic Biol Med

GPx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum.

Authors - Thomas Ramming
humanWB

Carcinogenesis

Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesi.

Authors - Dun-Fa Peng
  • KD Validated