GPX7 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPX7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 13501-1-AP

Synonyms

CL683, glutathione peroxidase 7, GPx 7, GPX6, GPX7, GSHPx 7

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Validation Data Gallery

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  WB analysis using 13501-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13501-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13501-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 13501-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13501-1-AP (GPX7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13501-1-AP (GPX7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human liver tissue, human placenta tissue, human brain tissue, Transfected HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

13501-1-AP targets GPX7 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPX7 fusion protein Ag4328
• Full Name: glutathione peroxidase 7
• Calculated molecular weight: 187 aa, 21 kDa
• Observed molecular weight: 18-21 kDa
• GenBank accession number: BC032788
• Gene symbol: GPX7
• Gene ID (NCBI): 2882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330). This antibody can ve detected as 18-21kDa due to signal peptide splicing.

Publications

Species	Application	Title
human	WB	Oncotarget; Glutathione peroxidase 7 suppresses cancer cell growth and is hypermethylated in gastric cancer.; Authors - Zheng Chen
chicken	IHC	Poult Sci; The development of antioxidant system in the intestinal tract of broiler chickens.; Authors - Dazhi Tang
human	WB	J Cancer; Glutathione Peroxidase 7 Suppresses Bile Salt-Induced Expression of Pro-Inflammatory Cytokines in Barrett's Carcinogenesis.; Authors - Dun-Fa Peng
human	WB	BMB Rep; GPx7 ameliorates non-alcoholic steatohepatitis by regulating oxidative stress.; Authors - Hyeon Ju Kim
human,mouse	WB,IHC	J Cell Mol Med; GPX7 Facilitates BMSCs Osteoblastogenesis via ER Stress and mTOR Pathway.; Authors - Xuchen Hu ; KD Validated
human	WB,IF	Front Oncol; GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma.; Authors - Yan Zhou ; KD Validated