GRAMD3 Polyclonal antibody

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• GRAMD3 Polyclonal antibody (25545-1-AP)

GRAMD3 Polyclonal Antibody for IHC, ELISA

Cat No. 25545-1-AP Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

GRAM domain-containing protein 2B, GRAMD2B, HCV NS3-transactivated protein 2, NS3TP2

• 

  IHC staining of human placenta using 25545-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25545-1-AP (GRAMD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 25545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25545-1-AP (GRAMD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 25545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25545-1-AP (GRAMD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: mouse kidney tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25545-1-AP targets GRAMD3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22226

  Product name: Recombinant human GRAMD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 252-432 aa of BC008590

  Sequence: SDLDGVVQQRRQDMEGYSSSGSQTPESENSRDFHATESQTVLNVSKGEAKPTRADAHVNRVPEGKAKSLPVQGLSETVGILHKVKSQKCPMLHHILIFYAIVVCALIISTFYMRYRINTLEEQLGLLTSIVDTHNTEQAAPSGLRSQVQFNVEVLCQELTANIVKLEKIQNNLQKLLENGD

• Full Name: GRAM domain containing 3
• Calculated Molecular Weight: 432 aa, 48 kDa
• GenBank Accession Number: BC008590
• Gene Symbol: GRAMD3
• Gene ID (NCBI): 65983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96HH9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRAMD3 is also known as GRAMD2B, as the human orthologs of the yeast Ltc/Lam proteins. The 432-amino acid GRAMD2B protein contains an N-terminal GRAM domain and a C-terminal transmembrane domain(PMID: 29469807). GRAMD2B localizes at contact sites between the endoplasmic reticulum (ER) and plasma membrane (PM) and forms homomeric complexes or heteromeric complexes with other GRAMD proteins(PMID: 31724953).