GRAP Polyclonal antibody

• Home
• Products
• Primary Antibodies
• GRAP Polyclonal antibody (14505-1-AP)

GRAP Polyclonal Antibody for IHC, ELISA

Cat No. 14505-1-AP Copied!

Publications (3)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

GRAP, GRB2 related adapter protein, GRB2 related adaptor protein

• 

  WB analysis of U-937 using 14505-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14505-1-AP (GRAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 14505-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14505-1-AP (GRAP antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 14505-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14505-1-AP (GRAP antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for GRAP in ?

Chat with Able

---

Tested Applications

• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14505-1-AP targets GRAP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5962

  Product name: Recombinant human GRAP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-217 aa of BC063035

  Sequence: MESVALYSFQATESDELAFNKGDTLKILNMEDDQNWYKAELRGVEGFIPKNYIRVKPHPWYSGRISRQLAEEILMKRNHLGAFLIRESESSPGEFSVSVNYGDQVQHFKVLREASGKYFLWEEKFNSLNELVDFYRTTTIAKKRQIFLRDEEPLLKSPGACFAQAQFDFSAQDPSQLSFRRGDIIEVLERPDPHWWRGRSCGRVGFFPRSYVQPVHL

• Full Name: GRB2-related adaptor protein
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC063035
• Gene Symbol: GRAP
• Gene ID (NCBI): 10750
• RRID: AB_2112704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13588
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The GRAP Polyclonal antibody (Cat# 14505-1-AP) has 3 total citations. These appear in Frontiers in Immunology (2025) and Life Sciences (2023, 2024). The research fields span tumor immunology (triple-negative breast cancer single-cell analysis), liver fibrosis regulation, and renal fibrosis in diabetic nephropathy. Applications cited include Western blot and immunofluorescence in human and mouse samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Front Immunol; Identification of tumor associated neutrophils-related genes in triple-negative breast cancer for predicting prognosis and therapeutic response through integrated single-cell analysis.; Authors - Koukou Li
mouse,human	WB	Life Sci; Grb2-related adaptor protein GRAP is a novel regulator of liver fibrosis; Authors - Xiaoyan Wu
mouse	WB	Life Sci; Ablation of Brg1 in fibroblast/myofibroblast lineages attenuates renal fibrosis in mice with diabetic nephropathy; Authors - Xiaoyan Wu