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GRB2 Monoclonal antibody

GRB2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1C5D11

Cat no : 66880-1-Ig

Synonyms

Adapter protein GRB2, ASH, EGFRBP GRB2, GRB2, Grb3 3, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66880-1-Ig targets GRB2 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRB2 fusion protein Ag0395
Full Name growth factor receptor-bound protein 2
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC000631
Gene symbol GRB2
Gene ID (NCBI) 2885
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-β protein precursor (AβPP). Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

Protocols

Product Specific Protocols
WB protocol for GRB2 antibody 66880-1-IgDownload protocol
IHC protocol for GRB2 antibody 66880-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

FASEB J

Low-magnitude vibration induces osteogenic differentiation of bone marrow mesenchymal stem cells via miR-378a-3p/Grb2 pathway to promote bone formation in a rat model of age-related bone loss.

Authors - Xiaoqin Yu
  • KD Validated